АТФазный домен в транспортных белках вирусов растений тема диссертации и автореферата по ВАК РФ 03.00.06, кандидат биологических наук Лещинер, Анна Дмитриевна

• Лещинер, Анна Дмитриевна
• кандидат биологических наук
• 2006

Оглавление диссертации кандидат биологических наук Лещинер, Анна Дмитриевна

Список сокращений.

Введение.

Обзор литературы:.

Многокомпонентные транспортные системы вирусов растений.

Транспорт вирусов с тройным блоком транспортных генов.

Особенности транспортной системы гордеи-подобных вирусов.

Особенности транспортной системы потекс-подобных вирусов.

Потивирусы.

Клостеровирусы.

Материалы и методы.

Результаты.

Обсуждение.

Выводы.

Заключение диссертации по теме «Вирусология», Лещинер, Анна Дмитриевна

Выводы

1. В составе НТФазно/хеликазных доменов ТБГ1 транспортных белков двух групп вирусов с тройным блоком транспортных генов - потексвируса X-вируса картофеля и гордеивируса полулатентного вируса мятлика выявлен минимальный субдомен, отвечающий за АТФазную активность белков.

2. Выявленный субдомен способен неспецифически и кооперативно взаимодействовать с РНК и образовывать гомоолигомеры.

3. Мутация консервативного остатка основной аминокислоты перед мотивом I не влияет на АТФазную активность минимального субдомена, но понижает эту активность полноразмерного НТФазно/хеликазного домена у вирусов обеих групп.

4. Рекомбинантный белок оболочки потивируса А-вируса картофеля, участвующего в транспорте вирусного генома, обладает Mg^-зависимой НТФазной активностью in vitro. Участок, отвечающий за данную активность, скорее всего, локализуется в С-концевой половине белка.

Благодарности

Искренне благодарю мою научную руковолительницу Наталию Олеговну Калинину, заведующего лабораторией Сергея Юрьевича Морозова, а также Андрея Геннадьевича Соловьева. Особая благодарность заведующему кафедрой вирусологии и отдела биохимии вирусов растений за предоставление возможности проведения данной работы. Благодарю всех сотрудников кафедры вирусологии за неоценимую помощь и поддержку во время выполнения данной работы. Отдельная благодарность Марине Владимировне Архипенко, Евгению Николаевичу Доброву, Роману Алексеевичу Зиновкину, Петру Алексеевичу Иванову, Татьяне Валерьевне Комаровой, Нине Иосифовне Луховицкой, Елене Алексеевне Мининой, Виктору Александровичу Новикову, Дарье Викторовне Ракитиной, Евгению Владимировичу Скурату, Татьяне Юрьевне Тилькуновой, Михаилу Вячеславовичу Щепетильникову.

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