Структура и некоторые биологические свойства липидов А из морских грамотрицательных бактерий родов Marinomonas и Chryseobacterium тема диссертации и автореферата по ВАК РФ 02.00.10, кандидат химических наук Воробьева, Елена Владимировна

• Воробьева, Елена Владимировна
• кандидат химических наук
• 2006

Оглавление диссертации кандидат химических наук Воробьева, Елена Владимировна

I. ВВЕДЕНИЕ.

II. ЛИТЕРАТУРНЫЙ ОБЗОР.

11.1. МЕХАНИЗМЫ ТОКСИЧЕСКОГО ДЕЙСТВИЯ ЭНДОТОКСИНОВ И СТРАТЕГИИ ПО ИХ НЕЙТРАЛИЗАЦИИ.

11.2. СТРУКТУРА ЛИПИДА А.

11.2.1. Общая характеристика липида А.

11.2.2. Варианты липида А, имеющие модификации в структуре гидрофильной части молекулы.

11.2.2.1. Вариации в углеводной части молекулы липида А.

11.2.2.2. Наличие остатков фосфорной кислоты.

11.2.3. Характеристика гидрофобной части молекулы липида А.

11.2.3.1. Качественный состав жирных кислот липида А.

11.2.3.2. Количественный состав жирных кислот липида А.

11.2 А. Бактерии, не содерэ/сащие липида А.

II.2.5. Взаимосвязь структуры и биологических свойств липида А.

III. РЕЗУЛЬТАТЫ И ОБСУЖДЕНИЕ.

111.1. ЛИПИДЫ А ИЗ БАКТЕРИЙ РОДА MARINOMONAS.

III. 1.1. Структура липида А из Marinomonas communis

АТСС 27118'.

III. 1.2. Структурная характеристика липида А из Marinomonas mediterránea АТСС 700492Т.

111.2. СТРУКТУРА ЛИПИДОВ А ИЗ БАКТЕРИЙ РОДА CHRYSEOBACTERIUM.

111.3. НЕКОТОРЫЕ ОСОБЕННОСТИ СОСТАВА НАРУЖНОЙ МЕМБРАНЫ

БАКТЕРИЙ РОДА CHRYSEOBACTERIUM.

III.3. НЕКОТОРЫЕ БИОЛОГИЧЕСКИЕ СВОЙСТВА ЛИПИДОВ А И ЛИПОПОЛИСАХАРИДОВ ИЗ БАКТЕРИЙ РОДОВ СHR YSEOBA CTERIUM И MARINOMONAS.

IV. ЭКСПЕРИМЕНТАЛЬНАЯ ЧАСТЬ.

V. ВЫВОДЫ.

Заключение диссертации по теме «Биоорганическая химия», Воробьева, Елена Владимировна

V. ВЫВОДЫ

1. Из морской грамотрицательной бактерии Marinomonas communis АТСС 27118Т выделен липид А необычной структуры - 1-фосфат (3-1'—»6-связанного дисахарида D-глюкозамина, ацилированного остатками редких короткоцепочечных (К)-3-додеканоилоксидекановой (или (Л)-З-деканоилоксидекановой) и (У^)-З-гидроксидекановой кислот в положениях 2 и 3 соответственно и (i?)-3-{(i?)-3-гидроксидеканоил}оксидекановой кислоты в положении 2'.

2. Липид А из бактерий М. mediterránea АТСС 700492 не содержит нормальных жирных кислот и представляет собой 1,4'-дифосфат глюкозаминобиозы, ацилированный остатками (Я)-3-{(/?)-3-гидроксидеканоил}оксидекановой кислоты в положениях 2, 2' и (К)~ 3-гидроксидекановой кислоты в положении 3. Липиды А бактерий рода Marinomonas -первые представители соединений этого класса, содержащие необычную 3-ацилоксиалкановую кислоту, состоящую из двух остатков (Я)-З-гидроксидекановой кислоты.

3. Впервые из диких, не мутантных, штаммов морских бактерий Chryseobacterium in

Т Т doltheticum CIP 103168 и С. scophthalmum CIP 104199 выделен липид А моносахаридной природы: 1-фосфат £)-глюкозамина, ацилированный остатками (/?)-3-гидрокси-изо-пентадекановой и (^)-З-гидрокси-шо-гептадекановой кислот в положениях 2 и 3 соответственно.

4. Показано, что липиды А из бактерий М. communis, М. mediterránea и С. indoltheti-сит, а также липополисахарид из М. communis проявляют низкую токсичность.

5. Липиды А из М. communis и С. indoltheticum, а также липоплисахарид из М. communis не индуцируют синтез фактора некроза опухоли альфа клетками периферической крови человека при всех изученных концентрациях. Липиды А из М communis и С. indoltheticum в дозе 10000 нг/мл ингибируют индуцированную липополисахаридом из Escherichia coli продукцию фактора некроза опухоли альфа на 50 %. Липополисахарид из М. communis в концентрации 1000 нг/мл ингибирует индуцированный синтез фактора некроза опухоли альфа на 80 % и является антагонистом эндотоксинов универсального типа действия.

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