Изучение регуляции транскрипции генов бактериофагов во время инфекции хозяйской клетки тема диссертации и автореферата по ВАК РФ 03.01.03, кандидат биологических наук Климук, Евгений Иванович

• Климук, Евгений Иванович
• кандидат биологических наук
• 2013

Оглавление диссертации кандидат биологических наук Климук, Евгений Иванович

Обозначения и сокращения.

Общая характеристика работы.

Актуальность проблемы.

Цели работы.

Научная новизна.

Практическая значимость работы.

Публикации и апробация работы.

Литературный обзор.

Заключение диссертации по теме «Молекулярная биология», Климук, Евгений Иванович

Выводы

1) Показана функциональная активность in vivo трех временных классов промоторов бактериофага Т5. Идентифицировано два промотора, активных на предранней стадии инфекционного цикла, восемь ранних промоторов и три поздних промотора. Показано, что ранние промоторы бактериофага Т5 содержат идентичную последовательность 5'-АТАТТ-3' между «-10»-элементом и стартовой точкой транскрипции.

2) Показано, что мутации в генах D5, D15 и С2 бактериофага Т5 не влияют на профиль транскрипции генов фага. Продукты генов Al и А2 необходимы для ингибирования транскрипции предранних генов.

3) В препаратах РНКП, выделенных из клеток, инфицированных фагом Т5, обнаружен белок gp26. gp26 прочно связывается с РНКП Е. coli (Кд < 1 нМ). Взаимодействие с РНКП происходит за счет связывания с участками ß-субъединицы, расположенными между аминокислотными остатками 1-151 и 703-795. gp26 ингибирует взаимодействие о70-холофермента РНКП с дискриминаторным участком промотора. В ходе инфекции gp26 необходим для оптимального ингибирования транскрипции ранних и, в меньшей степени, предранних генов и стимуляции синтеза поздних транскриптов.

4) У фКМУ-подобных бактериофагов Pseudomonas биоинформатически предсказаны гомологи ингибитора бактериальной РНКП - белка gp2 бактериофага Т7. Функциональный анализ in vivo и in vitro подтвердил гомологию gp2-noflo6Hbix белков фКМУ-подобных бактериофагов и белка gp2 бактериофага Т7.

Список работ, опубликованных по теме диссертации

Тезисы конференций:

1) Климук Е И Изучение регуляции экспрессии генов бактериофагов Е coli Т5 и phiEco32 для поиска антибактериальных препаратов Научно-техническая конференция «Биотехнологические аспекты фундаментальной науки БиоНаноскопия» УРАН ИБГ РАН, Москва 2010

2) Е Klimuk, N Akulenko and К Sevennov Regulation of gene expression of bacteriophage T5 revisited International conference "Viruses of Microbes", 2010, Pans, France

3) E Klimuk, N Akulenko and К Sevennov Regulation of gene expression of bacteriophage T5 International conference "Microbial Viruses Genomics, Evolution and Applications in Ecology, Biotechnology and Medicine", 2011, Belfast, UK

4) E Klimuk, N Akulenko and К Sevennov Regulation of gene expression of bacteriophage T5 FEMS Congress'11, 2011, Geneva, Switzerland

5) E Klimuk, N Akulenko, К Makarova, К Sevennov Identification and characterization of host RNA polymerase inhibitors encoded by phages of phiKMV-group International conference "Viruses of Microbes", 2012, Brussels, Belgium

6) E Klimuk, К Sevennov Identification and characterization of host RNA polymerase inhibitors encoded by phages of phiKMV-group International conference "Postgenome 2012", 2012, Kazan, Russia

Публикации в журналах:

7) Pavlova, О , Lavysh, D , Klimuk, E , Djordjevic, M , Ravcheev, D A , Gelfand, M S , Sevennov, К , Akulenko, N , Temporal regulation of gene expression of the Escherichia coli bacteriophage phiEco32 J Mol Biol, 2012 416(3) p 389-99

8) Evgeny Klimuk, Natalia Akulenko, Kira S Makarova, Pieter-Jan Ceyssens, Ivan Volchenkov, Rob Lavigne, Konstantin Sevennov Host RNA polymerase inhibitors encoded by phiKMV-like phages of Pseudomonas Virology 436 (2013), pp 67-74

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