Характеристика транскрипции в митохондриях жгутиковых простейших тема диссертации и автореферата по ВАК РФ 03.00.03, кандидат биологических наук Бессолицына, Екатерина Андреевна

• Бессолицына, Екатерина Андреевна
• кандидат биологических наук
• 2004

Оглавление диссертации кандидат биологических наук Бессолицына, Екатерина Андреевна

Введение.

Обзор литературы.

Структура митохондриального генома различных организмов.

Инициация митохондриальной транскрипции.

Инициация транскрипции животных.

Инициация транскрипции в митохондриях беспозвоночных животных.

Инициация транскрипции в митохондриях грибов.

Инициация транскрипции в митохондриях растений.

Транскрипция митохондриальной ДНК у различных групп организмов.

Процессинг митохондриальных пре-мРНК у различных групп организмов.

Нарезание полицистронных пре- мРНК, сигналы процессинга.

Модификация З'-конца митохондриальных мРНК.

Сплайсинг митохондриальных интронов.

Редактирование митохондриальных мРНК.

Структура и экспрессия митохондриального генома у представителей отряда

Kinetoplastida.

Организация митохондриального генома.

Транскрипция митохондриального генома представителей семейства

Trypanosomatidae.

Процессинг митохондриальных пре-мРНК у представителей отряда Kinetoplastida.

Уридиловое редактирование пре-мРНК.

Нарезание и сигналы процессинга пре-мРНК в митохондриях трипаносоматид.

Материалы и методы.

Выделение суммарной и митохондриальной РНК.

Выделение кинетопластной ДНК.

Электрофоретические методы.

Northern-гибридизация.

Полимеразная цепная реакция (ПЦР).

3' RACE 12S и 9S рРНК.

5' RACE 12S и 9S рРНК.

Клонирование PCR-продуктов.

Выделение митохондрий Leptomonas seymouri.

Компьютерный анализ.

Результаты и обсуждение.

Транскрипция и созревание митохондриальных рРНК Leptomonas seymouri.

Транскрипция и процессинг белок-кодирующих генов максикольцевой ДНК

Leptomonas seymouri.

Выводы.

Заключение диссертации по теме «Молекулярная биология», Бессолицына, Екатерина Андреевна

Выводы.

1. а) В максикольцевой ДНК Leptomonas seymouri обнаружено 6 сайтов, способных инициировать транскрипцию, один из которых располагается в дивергентной области остальные 5 - в консервативной. в) анализ участков, способных инициировать транскрипцию показал, что все они содержат нуклеотидную последовательность АААААС(С/Т)(С/Т).

2. Показано, что с максикольцевой ДНК Leptomonas seymouri транскрибируется три полицистронные молекулы РНК (тетрацистронная, включающая продукты генов ND7, COIII, Cyb, А6; трицистронная - 12S рРНК, 9S рРНК, ND8; дицистронная -RPS12, ND5) и моноцистронная - ND4.

3. Редактирование мРНК COIII, Cyb и А6 осуществляется в составе полицистронной РНК. Для мРНК А6 показано наличие продуктов альтернативного редактирования.

4. Зрелые 12S рРНК и 9S рРНК не содержат модификаций на своих З'-концах, тогда как все обнаруженные нами пре-мРНК - полиаденилированы.

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144. А Е- 0.J и*1/ш>1 В Е--Ч Hal/malс Е--10.4 kkal/»ol1. D tf- -13.9 kk»J/MoI1. Е Г kk*lSnol