Исследование активности тобамовирусных участков внутренней инициации трансляции в различных растительных системах тема диссертации и автореферата по ВАК РФ 03.00.12, кандидат биологических наук Зверева, Светлана Дмитриевна

• Зверева, Светлана Дмитриевна
• кандидат биологических наук
• 2003

Оглавление диссертации кандидат биологических наук Зверева, Светлана Дмитриевна

Список используемых сокращений.

I. Введение.

II.Обзор литературы.

II. 1. Принципы инициации трансляции в растениях.

11.2. Сканирующая модель инициации трансляции.

II.2.1. Кэп- и поли(А)-зависимая инициация трансляции. 13 И.2.2. Инициаторный кодон и его контекст.

11.2.2.1. Механизм сканирования с пропусканием инициаторного код она.

11.2.2.2. Терминация - реинициация.

И.2.3. Влияние вторичной структуры.

II.2.3.1. Нелинейное сканирование (шунтирование).

11.3. Внутренняя инициация трансляции.

11.3.1. Фактор-зависимая внутренняя инициация трансляции.

11.3.2. Фактор-независимая внутренняя инициация трансляции.:.

11.3.3. Механизм комплементарного взаимодействия IRES и 18S рРНК.

11.4. Внутренняя инициация трансляции в растениях.

11.4.1. Внутренняя инициации трансляции, обеспечиваемая последовательностями из генома тобамовирусов.

11.4.2. Внутренняя инициация трансляции в растениях -биологическое значение.

III. Материалы и методы.

IV. Результаты и обсуждение.

IV. 1. Исследование активности IRES из РНК ВТМ U1 и кр

ВТМ в изолированных протопластах Nicotiana tabacum.

IV. 1.1. Трансляция бицистронных конструкций в протопластах N. tabacum.

IV. 1.2. Экспрессия в протопластах моноцистронных конструкций "Ш-IRES-GUS".

IV. 1.3. Исследование делеционных мутантов IRES

1 48бокр"в™ в растительных протопластах.

IV.2. Изучение активности IRES-последовательностей из генома кр-ВТМ в трансгенных растениях N. tabacum.

IV.2.1. ПЦР-анализ трансгенных растений.

IV.2.2. Анализ тотальной РНК выделенной из трансгенных растений.

IV.2.3. Исследование экспрессии первого гена и оценка эффективности работы различных IRES в растениях.

IV.3. Исследование влияния области внутренней посадки рибосом на инфекционность ВТМ.

V. Выводы.

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