Иммунофенотипические и морфо-функциональные особенности активированных лимфоцитов тема диссертации и автореферата по ВАК РФ 14.00.36, кандидат биологических наук Карамзин, Андрей Михайлович

• Карамзин, Андрей Михайлович
• кандидат биологических наук
• 2008

Оглавление диссертации кандидат биологических наук Карамзин, Андрей Михайлович

Введение.

1.Обзор литературы.

2.Материалы и методы.

3. Результаты исследования.

I I г

3.1 Морфо-функциональная и иммунофенотипическая характеристика лимфокин активированных киллеров, генерированных из мононуклеарных лейкоцитов периферической крови доноров.

3.1.1 Морфологическая характеристика мононуклеарных лейкоцитов периферической крови доноров при активации ИЛ-2.

3.1.2 НК-активность лимфокин активированных киллеров.

3.1.3 Цитотоксическая активность лимфокин активированных киллеров по отношению к опухолевым и ^трансформированным линиям различного гистогенеза.

3.20ценка иммунофенотипа лимфокин активированных киллеров, генерированных из мононуклеарных лейкоцитов периферической крови доноров.

З.ЗКомбинированное действие ЛАК и цисплантина на клетки немелкоклеточного рака легкого.

3.4 Генерация ЛАК из МЛ селезенки и опухолевого экссудата онкологических больных.

3.4.1 Получение лимфокин активированных киллеров из лимфоцитов селезенки онкологических больных.

3.4.2 Генерация лимфокин активированных киллеров из мононуклеарных лейкоцитов опухолевых выпотов.

3.5 Цитотоксическая активность МНК при воздействии иммуномодуляторов различной природы.

Обсуждение результатов.

Выводы.

Заключение диссертации по теме «Аллергология и иммулология», Карамзин, Андрей Михайлович

Выводы

1.Оптимальным сроком для генерации ЛАК является 3-5 сут., в этот период появляются активированные формы типа иммунобластов и пролимфоцитов. Последующее культивирование приводит к увеличению содержания в культурах МЛ клеток макрофагального ряда.

2. Установлено, что лимфокин-активированные лимфоциты характеризуются более высокой пролиферативной и НК-активностыо, и отличаются высоким уровнем экспрессии молекул адгезии (CD58), активационных антигенов (CD38, CD25) и маркеров НК (CD16, CD56).

3. МНК и ЛАК обладают избирательной цитотоксической активностью по отношению к опухолевым клеточным линиям различного генеза и практически не оказывают цитопатогенного действия на нетрасформированные клетки.

4. ЛАК способны эффективно лизировать клетки немелкоклеточного рака легкого, устойчивые к действию препаратов платины.

5. ЛАК, полученные из мононуклеарных лейкоцитов селезенки и злокачественных выпотов онкологических больных, характеризуются высоким содержанием НК и обладают способностью эффективно лизировать аллогенные и аутологичные опухолевые клетки.

6. Показано, что иммуномодуляторы различной, в том числе и нецитокиновой природы, достоверно повышают спонтанную НК- активность и цитотокчиность лимфоцитов по отношению к опухолевым клеткам.

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